Coding

Part:BBa_K2223001:Design

Designed by: Thomas Sousa   Group: iGEM17_UCSC   (2017-11-01)


nhoA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 846
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence had to be codon optimized for use in Synechococcus elongatus PCC 7942. Homologous arms were added later for recombination into the genome of S. elongatus PCC 7942.


Source

Escherichia coli

References

J. C. Anderson, T. HSIAU, S. Srivastava, P. RUAN, J. P. I. KOTKER, R. BODIK, and S. A.Seshia, “Method for biosynthesis of acetaminophen,” May 2016. International ClassificationC12P13/02, C12N1/21; Cooperative Classification C12N9/1029, C12N9/0073, C12P13/02.